Assay Development
- In vitro Functional, and & Cell Based Assays
- Fluorescent & Luminescent Detection Technologies
- Assays for HTS and Compound Profiling
- Cloning Capabilities
- Cell Line Development (single-clone or pool)
High-Throughput Screening (uHTS)
- Collection of ~5.5 million compounds
- 1536-well assay exclusively (4-8 µL volume)
- All assays are off-bead – compound is already added into the wells
- Counter-screen assay on an active subset collection
Compound Profiling
- Can be in 384-well or 1536-well assay
- Generally in low-volume 384-well format (10-20 µL volume)
- Each compound gets a full 12-point concentration-dependence curve
- Selectivity vs. related proteins
- Ortholog in relevant species