Cell Line Development

GD³ is dedicated to delivering high-quality stable cell lines that accelerate your drug discovery and development program. Stable cell lines are essential tools in biotechnology and research, as they provide a consistent and reproducible system for various applications including assay development, studying cellular processes, drug interaction, protein production and disease modeling.

GD³ provides custom-built services to develop two types of genetically modified cell products, gene knockout lines (KO cell lines) and stable expression lines (Transgenic cell lines), which are generated by CRISPR gene editing or lentivector integration with parental mammalian cells, including cancer cells and immune cells.

With a commitment to innovation, quality, and collaboration, we partner with you to bring your project to fruition.

• Transgenic Cell Lines

  We routinely transduced different types of cells using lentivirus containing target genes and generated stable cell lines with varying expression levels. Either constitutive or inducible expression of target genes has been attained in isolated cell clones.

  As required, gene(s) of interest (or variant thereof) is (are) cloned into a lentivector and high-titer lentiviral particles are prepared. After infection and selection of primary cultures or established lines, stable cells will be tested for transgene expression and further processed for clonal isolation. Based on screening results, single clones with desired expression will be selected to make product stocks.

  • Regular characterization of cell cultures
    • Cell viability assay (Phenotypic analysis)
    • Cell-based reporter assay (luminescence and fluorescence microplate assay)
    • Mycoplasma qPCR assay (Mycoplasma infection diagnosis)
• Knockout/Knock-In Cell Lines

  We use state-of-the-art knockout reagents and the most efficient protocols to generate knock-out (KO) cell lines for different genes in mouse or human cell lines. Notably, a double-gene KO cell line has been created through two rounds of CRISPR editing. Edited cell pools and clones are evaluated by PCR or sequencing for identifying the genetic alteration and western blotting for protein expression level.

  

  Figure 1. Western blot analysis showing the successful removal of the PKR protein in cell lines through CRISPR-mediated gene editing
• Reporter Cell Lines

  GD³ harnesses the power of genetic engineering to create custom reporter cell lines that provide real-time insights into gene expression, signaling pathways, and cellular responses. Common reporter genes include luciferase, GFP, β-galactosidase, which produce easily quantifiable signals, allowing real time monitoring of biological processes. GD³ scientists can create custom reporter cell lines tailored to your specific needs for a wide range of applications including gene expression studies, drug screening and toxicology.

• Related Services:
  • Cell culture services
  • Cell line characterization
    • Short tandem repeats analysis
    • Virus detection in human cell lines
    • Transfection and transduction services
    • Assay development

Learn more about DMPK, LC-MS/MS, Immunoassays, qPCR, Biomarker Detection,