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Genesis Drug Discovery & Development
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Genesis Drug Discovery & Development is a proud member of Genesis Drug Discovery & Development (GD3), a fully integrated CRO providing services to support drug discovery programs of our clients from target discovery through IND filing and managing Phase I-III clinical trials.

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Biochemistry & Bioanalytical

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Ligand Binding Assays and Biomarker Detection

GD3's team of experienced scientists has partnered with clients worldwide to develop assays for a wide range of applications. Our biochemists have extensive experience developing and qualifying custom assays for the detection of large molecules, such as antibodies, nucleic acids, and biomarkers. We also work with commercially available kits and can develop fit-for-purpose assays.

GD3 uses Meso Scale Discovery (MSD) technology for these assays. The MSD platform, while similar to ELISA, uses electrochemiluminescence detection to analyze complex sample matrices for a variety of analytes.

  • Assay Characteristics:
    • Sensitivity pg/mL
    • Large dynamic range – pg/mL to µg/mL
    • Assay reproducibility
    • Quick turnaround time (2-3 hours) once assay is established
    • Minimal matrix effects
  • Ligand Binding Assays
    • ELISA
    • Meso Scale Discovery (MSD)
    • Oligonucleotide Hybridization Assays
    • Biomarker Detection
    • Custom Assay Development
  • Available Services:
    • Method transfer
    • Method development
    • Method qualification
    • Method validation (FDA GLP)
    • Sample analysis (clinical and non-clinical)
Figure 1. Standard Curve Using Recombinant Human Pax-6 protein in a custom MSD assay. Shown are data from runs conducted on four different days. Symbols represent duplicates (±range, error bars are smaller than symbols).
Reference:
  • Phillip G. Zaworski, Rachel Schwartz, et al. Quantitation of Pax-6 protein in ocular impression cytology samples using an electrochemiluminescence immunoassay, Analytical Biochemistry, Volume 656, 2022, 114876, ISSN 0003-2697, https://doi.org/10.1016/j.ab.2022.114876.

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